| 棘孢木霉丝裂原活化蛋白激酶基因task1的克隆及序列分析 |
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| 引用本文： | 杨萍,杨谦.棘孢木霉丝裂原活化蛋白激酶基因task1的克隆及序列分析[J].菌物研究,2012(4):228-230. |
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| 作者姓名： | 杨萍 杨谦 |
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| 作者单位： | 哈尔滨工业大学环境科学与工程系;哈尔滨商业大学食品科学与工程系 |
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| 基金项目： | 国家科技支撑计划子课题(2007BAD65B03-02);黑龙江省重大科技攻关项目(GA08B101-1) |
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| 摘 要： | 为深入研究丝裂原活化蛋白激酶(Mitogen-activated protein kinase,MAPK)的功能,从棘孢木霉(Tricho-derma asperellum)中克隆了丝裂原活化蛋白激酶(MAPK)基因task1,并对其序列进行分析。该基因编码355个氨基酸,全长1 757 bp,理论分子质量41.1 kD,理论等电点为6.64,与深绿木霉(T.atroviride)MAPK基因tmk1、里氏木霉(T.reesei)MAPK基因tmkA和绿色木霉(T.virens)MAPK基因tmkA在氨基酸和核苷酸水平上同源性都很高,蛋白结构预测为丝氨酸/苏氨酸蛋白激酶。
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| 关 键 词： | 棘孢木霉 丝裂原活化蛋白激酶 克隆 序列分析 |
Cloning and Sequence Analysis of Trichoderma asperellum Mitogen-Activated Protein Kinase(MAPK) Gene Task1 |
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| YANG Ping,YANG Qian.Cloning and Sequence Analysis of Trichoderma asperellum Mitogen-Activated Protein Kinase(MAPK) Gene Task1[J].Journal of Fungal Research,2012(4):228-230. |
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| Authors: | YANG Ping YANG Qian |
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| Institution: | 1(1.Department of Environmental Science and Engineering,Harbin Institute of Technology,Harbin 150050,China;2.Department of Food Science and Engineering,Harbin University of Commerce,Harbin 150050,China) |
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| Abstract: | We cloned and analyzed mitogen-activated protein kinase （MAPK） gene taskl from Trichoder- ma asperellum for further studying the mitogen-activated protein kinase （MAPK） function. The whole length of the gene taskl was 1 757 bp, encoding 355 amino acids, theoretical molecular weight was 41.1 kD and theoretical isoelectric point was 6.64. The gene taskl has high homology with Tr/choderma atroviride MAPK gene tmkl, Tr/ehoderma reesei MAPK gene trnkA and Trichoderma virens MAPK gene tmkA in amino acid and nucleotide level. The protein structure prediction of gene taskl was a serine/ threonine protein kinase. |
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| Keywords: | Trichoderma asperellum mitogen-activated protein kinase cloning sequence analysis |
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