|
|||||
|
|
| 离褶伞属菌株酯酶同工酶分析 | |
| 引用本文： | 李胜南,宋慧,隋玉龙,李媛媛,潘祖桐,李晓.离褶伞属菌株酯酶同工酶分析[J].菌物研究,2013,11(1):38-44. |
| 作者姓名： | 李胜南 宋慧 隋玉龙 李媛媛 潘祖桐 李晓 |
| 作者单位： | 1. 吉林农业大学生命科学学院,长春,130118 2. 吉林农业大学生命科学学院,长春130118;吉林农业大学教育部食药用菌工程研究中心,长春130118 3. 吉林农业大学菌物研究所,长春,130118 |
| 基金项目： | 吉林省世行贷款农产品质量安全地方标准制修订项目(2013) |
| 摘 要： | 对45个离褶伞属真菌菌株进行酯酶同工酶聚丙烯酰胺凝胶电泳(PAGE)分析,分析其酶谱多态性,并采用聚类分析方法对菌株间的亲缘关系进行鉴定。结果表明:在45个离褶伞属菌株酯酶同工酶酶谱中,共检测到了10条相对迁移率不同的酶带,多态性较高,并且在相似系数为0.75时,可将供试菌株分为4类,第一类包括17个菌株,第二类包括16个菌株,第三类包括11个菌株,第四类包括1个菌株。 |
| 关 键 词： | 离褶伞 酯酶同工酶 聚丙烯酰胺凝胶电泳 聚类分析 |
Esterase Isozyme Analysis of Lyophyllum Strains |
|
| LI Sheng-nan,SONG Hui,SUI Yu-long,LI Yuan-yuan,PAN Zu-tong,LI Xiao.Esterase Isozyme Analysis of Lyophyllum Strains[J].Journal of Fungal Research,2013,11(1):38-44. | |
| Authors: | LI Sheng-nan SONG Hui SUI Yu-long LI Yuan-yuan PAN Zu-tong LI Xiao |
| Institution: | 1.College of Life Sciences,Jilin Agricultural University,Changchun 130118,China;2.Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University,Changchun 130118,China;3.Mycology Institute of Jilin Agricultural University,Changchun 130118,China) |
| Abstract: | Polyacrylamide gel electrophoresis（PAGE） and cluster analysis were used to analyse the esterase zymogram polymorphism and genetic relationships of 45 Lyophyllum Karst.strains.The results showed that 10 different bands were detected among 45 strains.Cluster analysis results suggested that 45 strains could be divided into 4 major groups at the similarity coefficient of 0.75,Group Ⅰ includes 17 strains,Group Ⅱ includes 16 strains,Group Ⅲ includes 16 strains,the fourth includes 1 strain. |
| Keywords: | Lyophyllum esterase isozyme polyacrylamide gel electrophoresis cluster analysis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |