| 一种简单的竹黄菌(Shiraia bambusicola)单孢分离方法 |
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| 引用本文： | 刘永翔,朱国胜,刘作易.一种简单的竹黄菌(Shiraia bambusicola)单孢分离方法[J].菌物研究,2008,6(1):49-51. |
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| 作者姓名： | 刘永翔 朱国胜 刘作易 |
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| 作者单位： | 1. 贵州省农业生物技术重点实验室,贵阳,550006;贵州省农业科学院生物技术研究所,贵阳,550006
2. 贵州省农业生物技术重点实验室,贵阳,550006 |
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| 基金项目： | 贵州省农业科学院资助项目 |
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| 摘 要： | 介绍了一种简单、快速的利用毛细管分离竹黄单孢菌株的方法。用毛细管吸有孢子液一端的轻印在固体培养基表面的印迹固定显微视野,快速确定单孢,简易地分离了竹黄单孢菌株。该方法值得推广到其它种类真菌的单孢分离工作中。
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| 关 键 词： | 竹黄 毛细管 单孢分离 |
| 文章编号： | 1672-3538(2008)01-0049-02 |
| 修稿时间： | 2007年10月13 |
A Simple Method for Obtaining Single-Spore Isolates of Shiraia bambusicola |
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| LIU Yong-xiang,ZHU Guo-sheng,LIU Zuo-yi.A Simple Method for Obtaining Single-Spore Isolates of Shiraia bambusicola[J].Journal of Fungal Research,2008,6(1):49-51. |
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| Authors: | LIU Yong-xiang ZHU Guo-sheng LIU Zuo-yi |
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| Institution: | LIU Yong-xiang, ZHU Guo-sheng, LIU Zuo-yi(1. Guizhou Key Laboratory of Agricultural Biotechnology, Guiyang 550006, China; 2. Institute of Biotechnology , Guizhou Agricultural Academy of Sciences, Guiyang 550006, China ) |
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| Abstract: | A new simple and quick method with capillary to isolate single-spore strains of Shiraia bambusicola was described. A visual field under microscope was fixed by using a section of capillary which contains spore suspension to stamp on to the surface of a piece of medium, and single-spore isolates were obtained easily after quick identification of single spore in the fixed visual field under microscope. It is significant to enlarge application of this method in other species of fungi. |
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| Keywords: | Shiraia bambusicola capillary single-spore isolation |
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