| 喇叭石蕊共生菌藻液体培养的研究(英文) |
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| 引用本文： | 苏敏,魏江春.喇叭石蕊共生菌藻液体培养的研究(英文)[J].菌物研究,2008,6(1):57-62. |
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| 作者姓名： | 苏敏 魏江春 |
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| 作者单位： | 1. 河北大学生命科学学院,保定,071002
2. 河北大学生命科学学院,保定,071002;中国科学院微生物研究所真菌地衣系统学重点实验室,北京,100101 |
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| 摘 要： | 对喇叭石蕊共生菌、藻液体培养条件进行了研究。结果表明:共生菌生长在以40g/L肌醇为碳源、2g/LL-谷氨酰胺为氮源、起始pH值为7.0的LB液体培养基中,培养温度为20℃时表现最佳。其共生藻的生长在以160g/L葡萄糖为碳源、1.75g/LNaNO3为氮源、起始pH值为5.0的BBM液体培养基中,培养温度为20℃时表现最佳。
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| 关 键 词： | 地衣 喇叭石蕊 共生菌 共生藻 液体培养 |
| 文章编号： | 1672-3538(2008)01-0057-06 |
| 修稿时间： | 2007年6月18日 |
Research on Liquid Culture of Mycobiont and Photobiont Isolated from Cladonia pyxidata |
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| SU Min,WEI Jiang-chun.Research on Liquid Culture of Mycobiont and Photobiont Isolated from Cladonia pyxidata[J].Journal of Fungal Research,2008,6(1):57-62. |
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| Authors: | SU Min WEI Jiang-chun |
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| Institution: | SU Min , WEI Jiang-chun( 1. College of Life Science, Hebei University, Baoding 071002, China ; 2. Key Laboratory of Systematic Mycology ＆ Lichenology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China ) |
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| Abstract: | This paper deals with the cell growth of both the mycohiont and photobiont of Cladonia pyxidata collected from China in liquid culture.The experiments results showed that the better conditions of mycobiontic growth were cultured by using liquid Lilly-Barnett medium containing 40 g/L inositol as a carbon source,2 g/L L-glutamine as a nitrogen source,adjusting pH to 7.0 before autoclaving,and incubating cultures at 20℃.The better conditions of photobiontic growth were cultured by using liquid Bold's basal medium containing 160 g/L glucose as a carbon source,1.75 g/L NaNO3 as a nitrogen source,adjusting pH to 5.0 before autoclaving,and incubating cultures at 20℃. |
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| Keywords: | lichen Cladonia pyxidata mycobiont photobiont liquid culture |
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