| 皮下盘菌属及其近似类群RAPD-PCR反应条件的优化 |
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| 引用本文： | 何宇峰,王士娟,叶光斌,林英任.皮下盘菌属及其近似类群RAPD-PCR反应条件的优化[J].菌物研究,2005,3(2):1-5. |
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| 作者姓名： | 何宇峰 王士娟 叶光斌 林英任 |
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| 作者单位： | 1. 安徽农业大学林学与园林学院,安徽,合肥,230036
2. 安徽农业大学生命科学学院,安徽,合肥,230036 |
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| 基金项目： | 国家自然基金资助项目（30370011,3470014） |
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| 摘 要： | 以悬钩子皮下盘菌等总基因组DNA为皮下盘菌属(Hypodermade Not.)及其近似类群RAPD体系优化的模板,对模板DNA浓度、dNTPs浓度、Mg2+浓度、引物浓度、TaqDNA聚合酶浓度等反应体系以及退火温度和时间、延伸时间、循环次数等扩增程序条件进行优化,寻找出适合此类菌物RAPD-PCR反应的最佳反应体系和最佳扩增程序。
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| 关 键 词： | 皮下盘菌属 齿裂菌属 散斑壳属 RAPD-PCR反应条件 优化 |
| 文章编号： | 1672-3538（2005）02-0001-05 |
| 收稿时间： | 2005-03-07 |
| 修稿时间： | 2005年3月7日 |
Optimization of RAPD-PCR Reaction Conditions for Hypoderma and Its Similar Groups |
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| HE Yu-feng,WANG Shi-juan,YE Guang-bin,LIN Ying-ren.Optimization of RAPD-PCR Reaction Conditions for Hypoderma and Its Similar Groups[J].Journal of Fungal Research,2005,3(2):1-5. |
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| Authors: | HE Yu-feng WANG Shi-juan YE Guang-bin LIN Ying-ren |
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| Institution: | 1. College of Forest and Gardening, Anhui Agricultural University, Hefei, Anhui 230036, China; 2. College of Life Science, Anhui Agricultural University, Hefei, Anhui 230036, China |
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| Abstract: | Genomic DNA of Hypoderma rubi was regarded as template optimized RAPD system of Hypoderma and its similar groups.The influencing factors and experimental parameters on RAPD were studied,including templates,dNTPs,Mg2+,primers,Taq DNA polymerase,annealing temperature and time,elongation time and cycle numbers.Optimal reaction system and amplification procedure were selected to apply to RAPD-PCR reaction of Hypoderma and its similar groups. |
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| Keywords: | Hypoderma Coccomyees Lophodermium reaction condition of RAPD-PCR optimization |
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