Radiosensitization mechanism of riboflavin in vitro |
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Authors: | Guanshu Liu Changyuan Lu Side Yao Fang Zhao Yu Li Xiangshun Meng Jianguo Gao Jianming Cai Liming Zhang and Zhilong Chen |
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Institution: | 1. Laboratory of Radiation Chemistry, Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China 2. Laboratory of Radiation Medicine, The Second Military Medical University, Shanghai 200433, China 3. Laboratory of Toxicon & Pharmacy, The Second Military Medical University, Shanghai 200433, China |
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Abstract: | Riboflavin, suggested to be a radiosensitizer, was studied in murine thymocytes and human hepatoma L02 cell line in vitro with MTT method and fluorescence microscopy. When the murine thymocytes treated with 5–400 μmol/L riboflavin were irradiated
by 5 Gy 60Co γ ionizing radiation, the low concentration groups, i.e. treated with 5–50 μmol/L riboflavin, showed a different surviving
fractions-time relating correlation compared with the high concentration groups, i.e. treated with 100–400 μmol/L riboflavin.
The former had a high survival level at the end of irradiation, but which, after 4-h incubation, decreased rapidly to a low
level. On the contrary, the high concentration groups showed a low survival level at the end of irradiation, and a poor correlation
was found between the surviving fraction and the incubation time, after 4 h a little difference was observed. The results
of fluorescence microscopy indicated that under low concentration conditions, the riboflavin localized mainly in nucleus (both
perinuclear area and inside of nuclear membrane), while under high concentration conditions, intensive riboflavin also localized
around cytoplasmic membranes. Thus we can conclude: the riboflavin had radiosensitivity effect on DNA under low concentration
conditions, and enhanced the damage to cytoplasmic membrane under high concentration conditions. Also the most effective concentration
of riboflavin can be evaluated to be approximate 100 μmol/L. |
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Keywords: | riboflavin murine thymocyte L02 cell MTT method fluorescence microscopy radiosensitization |
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