Use of the modified viral satellite DNA vector to silence mineral nutrition-related genes in plants: silencing of the tomato ferric chelate reductase gene, <Emphasis Type="Italic">FRO1</Emphasis>, as an example |
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| Authors: | XiuXia He ChongWei Jin GuiXin Li GuangYi You XuePing Zhou ShaoJian Zheng |
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| Institution: | 1.Key Laboratory for Environmental and Ecosystem Health of the Ministry of Education, College of Environmental and Resource Science,Zhejiang University,Hangzhou,China;2.College of Life Science,Changchun University of Science and Technology,Changchun,China;3.Institute of Biotechnology,Zhejiang University,Hangzhou,China;4.State Key Laboratory of Plant Physiology and Biochemistry, College of Life Science,Zhejiang University,Hangzhou,China |
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| Abstract: | Virus-induced gene silencing (VIGS) is potentially an attractive reverse-genetics tool for studies of plant gene function,
but whether it is effective in silencing mineral nutritional-related genes in roots has not been demonstrated. Here we report
on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmβ) associated with Tomato
yellow leaf curl China virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FRO1) from tomato was inserted into the DNAmβ vector. Tomato roots agro-inoculated with DNAmβ carrying both a fragment of FRO1 and TYLCCNV used as a helper virus exhibited a significant reduction at the FRO1 mRNA level. As a consequence, ferric chelate reductase activity, as determined by visualization of the pink FeBPDS3 complex was significantly decreased. Our results clearly demonstrated that VIGS system can be employed to investigate gene
function associated with plant nutrient uptake in roots. |
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