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Map-based cloning of the <Emphasis Type="Italic">ALK</Emphasis> gene,which controls the gelatinization temperature of rice
Authors:Zhenyu?Gao  Dali?Zeng  Xia?Cui  Yihua?Zhou  Meixian?Yan  Danian?Huang  Jiayang?Li  Email author" target="_blank">Qian?QianEmail author
Institution:1. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;National Center for Plant Genome Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
2. State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
3. National Center for Plant Genome Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
Abstract:Gelatinization temperature (GT) is an important parameter for evaluating the cooking and eating quality of rice besides amylose content (AC). The inheritance of the genes affecting GT has been widely studied and is considered to be controlled by a major gene. Here, we report the map-based cloning of rice ALK that encodes the soluble starch synthase II (SSSII). Comparison between the DNA sequences from different rice varieties, together with the results obtained with digestion of the rice seeds in alkali solution, indicates that the base substitutions in coding sequence of ALK may cause the alteration in GT.
Keywords:rice (Oryza sativa L  )  ALK  soluble starch synthase Ⅱ  gelatinization temperature  map-based cloning
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