Effect of recombinant growth hormone on expression of growth hormone receptor,insulin-like growth factor mRNA and serum level of leptin in growing pigs |
| |
Authors: | Qingfu?Xu Zhihui?Zhao Yingdong?Ni Email author" target="_blank">Ruqian?ZhaoEmail author Jie?Chen |
| |
Institution: | 1.Laboratory of Animal Physiology & Biochemistry, College of Animal Medicine,Nanjing Agricultural University,Nanjing,China |
| |
Abstract: | Sixteen Large White × Landrace castrated male pigs were allotted into treatment and control group. The treatment group was
injected intramuscularly with recombinant porcine growth hormone (rpGH, 4 mg d−1) and the control group with vehicle for 28 days. Animals were slaughtered 4 h after final injection for liver, longissimus
dorsi (LD) muscle and blood sampling. Serum concentration of insulin-like growth factor 1 (IGF-I) and leptin were determined
by RIA. The total RNA was extracted from tissues to measure the abundance of growth hormone receptor (GHR), IGF-I mRNA by
RT-PCR with 18S rRNA internal standard. Results showed that rpGH enhanced the average daily weight gain by 26.1% (P < 0.05), the serum IGF-I concentration by 70.94% (P < 0.01), decreased serum leptin by 34.8% (P < 0.01). The relative abundance of GHR and IGF-I mRNA in liver were increased by 24.45% (P < 0.05) and 45.30% (P < 0.01), respectively, but no difference of GHR (P > 0.05) and IGF-I mRNA (P > 0.05) in LD between GH treated and control group was found. These results suggest that rpGH can up-regulate hepatic GHR
and IGF-I gene expression and improve animal growth. However the effect of rpGH on GHR and IGF-I gene expression are tissue-specific. |
| |
Keywords: | pigs growth hormone insulin-like growth factor Ⅰ growth hormone receptor |
本文献已被 万方数据 SpringerLink 等数据库收录! |
|