Cloning of a NaCl-induced fructose-1, 6-diphosphate aldolase cDNA from<Emphasis Type="Italic">Dunaliella salina</Emphasis> and its expression in tobacco |
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Authors: | Xiaoning?Zhang Changfa?Lin Huoying?Chen Hao?Wang Zhicai?Qu Hongwei?Zhang Jianhong?Yao Email author" target="_blank">Daleng?ShenEmail author |
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Institution: | 1.Institute of Genetics, State Key Laboratory of Genetic Engineering,Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, School of Life Sciences, Fudan University,Shanghai,China;2.Agricultural College of Jiaotong University,Shanghai,China |
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Abstract: | Using Rapid Amplification of cDNA ends (RACE) technique, the full-length cDNA encoding a NaCl-induced fructose-1, 6-diphosphate
aldolase (DsALDP) was obtained. It was shown that the DsALDP had a relatively high homology (66%–73%) to chloroplast fructose-1, 6-diphosphate
aldolase (AldP) in many plants according to their amino acid sequences. The phylogenetic analysis further confirmed that AldP
in alga is the nearest to DsALDP. As to its expression pattern,DsALDP was denovo synthesized by NaCl induction. Its expression level was significantly changed with inducing time. After the selectedDsALDP cDNA subcloned into a binary vector pBI121, the new construct was introduced into tobacco byAgrobacterium tumefaciens. The results of Southern blot and RT-PCR analysis of four transgenic T1 plants indicated thatDsALDP was integrated into genome of these transgenic plants and effectively expressed. Aldolase activities have been detected in
T1-1, T1-2 and T1-3 plants by bioassay under 100–200 mmol/L NaCl. It was also observed that proline contents in them were
differentially increased. |
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Keywords: | D salina NaCl-induced differentially expressed chloroplast fructose-1 6-diphosphate aldolase salt tolerance |
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