Identification of salt-stress responsive genes in rice (Oryza sativa L.) by cDNA array |
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Authors: | Xinjian He Jianquan Chen Zhigang Zhang Jinsong Zhang Shouyi Chen |
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Institution: | Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, 100101, Beijing, China. |
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Abstract: | To identify salt stress-responsive genes, we constructed a cDNA library with the salt-tolerant rice cultivar, Lansheng. About 15000 plasmids were extracted and dotted on filters with Biomeck 2000 HDRT system or by hand. Thirty genes were identified to display altered expression levels responding to 150 mmol/L NaCI. Among them eighteen genes were up-regulated and the remainders down-regulated. Twenty-seven genes have their homologous genes in Gen-Bank Databases. The expression of twelve genes was studied by Northern analysis. Based on the functions, these genes can be classified into five categories, including photosynthesis-related gene, transport-related gene, metabolism-related gene, stress- or resistance-related gene and the others with various functions. The results showed that salt stress influenced many aspects of rice growth. Some of these genes may play important roles in plant salt tolerance. |
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Keywords: | cDNA array stress salt responsive gene rice |
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