RAI , one candidate gene associated with differentiation of human lung adenocarcinoma cells |
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Authors: | WANG Xuejiao ZHANG Rui LIU Zhihua WANG Xiuqin DING Fang GUO Mingzhou WU Min |
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Institution: | (1)National Laboratory of Molecular Oncology & Department of Cell Biology, Cancer Institute, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100021 ,China |
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Abstract: | From all-trans retinoic acid (ATRA)-treated human lung adenocarcinoma GLC-82 cells and control, subtractive cDNA library has been constructed using subtractive hybridization technique in our laboratory. The screening of the cDNA subtractive library resulted in identification of a clone containing cDNA fragment of one ATRA-induced gene (RAI) in GLC-82 cells. The positive clone with full-length cDNA of RAI was identified by screening fetal brain cDNA library using colony hybridization technique, and then sequenced. RT-PCR results showed that RAI was expressed in many different human fetal tissues. These results suggest that RAI may be involved in cell differentiation and play an important role in vital activities of cells. |
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Keywords: | lung adenocarcinoma differentiation retinoic acid RAI |
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