Crystal structure of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase |
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| Authors: | ZHU Xueyong GONG Weimin NIU Li wenTENG Maikun XU QingpingWU ChuanjinGUI Tao WANG YuzhenWANG Chun |
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| Institution: | 1.Young Scientist Laboratory of Structure Biology; Department of Biology; University of Science and Technology of China; Hefei 230026; China 2.State Key Laboratory of Bionmacromolecules; Institute of Biophysics; Chinese Academy of Sciences; Beijing 100101; China 3.Department of Biology; University of Science and Technology of China; Hefei 230026; China |
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| Abstract: | The crystal structures of Streptomyces diastaticus No. 7 strain M1033 xylose isomerase (SDXyI) have been analysed and refined at 0.19nm. The crystal space group is I222, with unit cell dimensions of a=9.884 ran, b=9.393nm and c=8.798nm. Based on the coordinates of the Streptomyces rubiginosus xylose isomerase (SRXyI), the initial model of SDXyl was built up by the dose packing analysing and R-factor searching and refined by PROLSQ to a final R-factor of 0.177 with the rms deviations of bond lengths and bond angles of 0.001 9nm and 2.1°, respectively. No significant global conformation change existed between SRXyI and SDXyI except the local conformation in the active site. |
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| Keywords: | xylose isomerase glucose isomerase streptomyces crystal structure |
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