The giant panda ( Ailuropoda melanoleuca ) somatic nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg |
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Authors: | Chen Dayuan Sun Qingyuan Liu Jilong Li Guangpeng Lian Li Wang Minkang Han Zhirning Song Xiangfen Li Jinsong Sun Qiang Chen Yucun Zhang Yaping and Ding Bo |
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Institution: | (1) State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100080 Beijing, China;(2) Fuzhou Giant Panda Research Center, 350001 Fuzhou, China;(3) Kunming Institute of Zoology, Chinese Academy of Sciences, 650223 Kunming, China |
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Abstract: | The giant panda skeletal muscle cells, uterus epithelial cells and mammary gland cells from an adult individual were cultured
and used as nucleus donor for the construction of intenpecies embryos by transferring them into enucleated rabbit eggs. All
the three kinds of somatic cells were able to reprogram in rabbit ooplasm and support early embryo development, of which mammary
gland cells were proven to be the best, followed by uterus epithelial cells and skeletal muscle cells. The experiments showed
that direct injection of mammary gland cell into enucleated rabbit ooplasm, combined within vim development in ligated rabbit oviduct, achieved higher blastocyst development thanin vitro culture after the somatic cell was injected into the perivitelline space and fused with the enucleated egg by electrical
stimulation. The chromosome analysis demonstrated that the genetic materials in reconstructed blastocyst cells were the same
as that in panda somatic cells. In addition, giant panda mitochondrial DNA (mtDNA) was shown to exist in the intenpecies reconstructed
blastocyst. The data suggest that (i) the ability of ooplasm to dedifferentiate somatic cells is not speciesspecific; (ii)
there is compatibility between intenpecies somatic nucleus and ooplasm during early development of the reconstructed egg.
Project supported by the 1998 Special Fund of the Chinese Academy of Sciences (KY95-J1-318) and a 1999 Special Fund from the
Ministry of Science and Technology. Instruments were dominated by Mr. Shum Yam Wa, Heal Force Development Ltd. |
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Keywords: | giant panda rabbit interspecies cloning dedifferentiation totipotency |
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