| Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts |
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| 作者姓名： | 卢萍 任民 翟中和 |
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| 作者单位： | LU Ping，REN Min & ZHAI ZhongheCollege of Life Sciences，Peking University，Beijing 100871，China |
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| 基金项目： | This work was supported by the National Natural Science Foundation of China (Grant Nos. 19890380 & 30070388)，the Special Funds for Major State Basic Research of China |
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| 摘 要： | The cell-free extracts from animal Xenopus laevis egg could induce chromatin decon-densation and pronuclear formation from demembranated plant (Orychophragmus violaceus) sperm. The demembranated Orychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network.
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Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts |
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| LU Ping,REN Min & ZHAI ZhongheCollege of Life Sciences,Peking University,Beijing ,China.Nuclear reconstitution of demembranated Orychophragmus violaceus sperm in Xenopus laevis egg extracts[J].Science China Life sciences,2002,45(6). |
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| Authors: | LU Ping REN Min & ZHAI ZhongheCollege of Life Sciences Peking University Beijing China |
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| Institution: | LU Ping,REN Min & ZHAI ZhongheCollege of Life Sciences,Peking University,Beijing 100871,China |
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| Abstract: | The cell-free extracts from animal Xenopus laevis egg could induce chromatin decon-densation and pronuclear formation from demembranated plant (Orychophragmus violaceus) sperm. The demembranated Orychophragmus violaceus sperm began to swell in 30 min incubation, and then were gradually decondensed. The reassembly of nuclear envelope in the reconstituted nuclei had been visualized by means of electron microscopy and fluorescent microscopy. Membrane vesicles fused to form the double envelope around the periphery of the decondensed chromatin. The morphology of the newly formed nucleus, with a double membrane, was similar to those nuclei after fertilization. Transmission electron microscope micrograph of the whole mount prepared nuclear matrix-lamina showed the reconstituted nucleus to be filled with a dense network. |
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| Keywords: | cell-free system nuclear reconstitution Orychophragmus violaceus demembranated sperm Xenopus laevis egg extracts |
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