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Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes
作者姓名:吕德扬  曹学远  唐顺学  田霞
作者单位:Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China  
摘    要:Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.

收稿时间:4 November 1999

Regeneration of foreign genes co-transformed plants ofMedicago sativa L byAgrobacterium rhizogenes
Deyang Lü,Xueyuan Cao,Shunxue Tang,Xia Tian.Regeneration of foreign genes co-transformed plants of Medicago sativa L by Agrobacterium rhizogenes[J].Science China Life sciences,2000,43(4):387-394.
Authors:Deyang Lü  Xueyuan Cao  Shunxue Tang  Xia Tian
Institution:(1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China
Abstract:Gene encoding sulphur amino acid-rich protein (HNP) and rol genes were transferred into Medicago sativa L (alfalfa) mediated by Agrobacterium tumafeciens. Regeneration of trans-genie plants was induced successfully from hairy root tissue of cotyledon in alfalfa. Cotyledon tissues were an ideally transformed recipient. There was a negative correlation between age of hairy roots and embryogenesis frequency in alfalfa. Production of co-transformed plants with greater yield and super quality was important for development of new alfalfa varieties.
Keywords:Medicago saliva  HNP gene  rol genes  co-transformed plant  
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