Resistance of SKW6 cell to apoptosis induction with anti-Fas antibody upon transduction of a reverse fragment to a cDNA encoding human 6A8 α-mannosidase |
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作者姓名: | 史耕先 靳玉兰 王壮志 崔巍 刘音 王讯 朱立平 |
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作者单位: | SHI Gengxian JIN Yulan WANG ZhuangzhiCUI Wei LIU Yin,WANG Xun& ZHU LipingInstitute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,China |
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基金项目: | the National Natural Science Foundation of China (Grant No. 39630300) and Chinese Academy of Medical Sciences. |
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摘 要: | The effect of transduction with a reverse fragment to a cDNA encoding human 6A8 α-mannosidase on apoptosis induction of human B cell line SKW6 by anti-Fas antibody was tested. Apoptosis-inducer of anti-Fas monoclonal antibody was used to induce apoptosis in SKW6 cells. Giemsa's staining, Annexin-V-FLUOS staining and DNA ladder test were used to determine the events of apoptosis. Indirect immunofluorescent staining with anti-Fas antibody was performed to detect the surface Fas expression. In a time-course test of 12, 24 and 36 h for apoptosis induction by anti-Fas antibody, DNA ladder was observed in the wild-type SKW6 cells in a time-dependent fashion. Mock transduction had no effect on DNA ladder production. However, no DNA ladder was detected in the rAAV-antisense 6A8 cDNA-transduced SKW6. Results from Annexin-V-FLUOS staining on anti-Fas antibody-treated cells revealed that the staining-positive rate in the rAAV-antisense 6A8 cDNA-transduced SKW6 cells was decreased in comparison to that in the wild-
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关 键 词: | apoptosis antisense 6A8 cDNA 6A8 α-mannosidase anti-Fas antibody B cell line SKW6. |
Resistance of SKW6 cell to apoptosis induction with anti-Fas antibody upon transduction of a reverse fragment to a cDNA encoding human 6A8 a-mannosidase |
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Authors: | SHI Gengxian JIN Yulan WANG ZhuangzhiCUI Wei LIU Yin WANG Xun& ZHU LipingInstitute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China |
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Institution: | SHI Gengxian JIN Yulan WANG ZhuangzhiCUI Wei LIU Yin,WANG Xun& ZHU LipingInstitute of Basic Medical Sciences,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100005,China |
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