| The effects of detergents DDM and β-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts |
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| 作者单位： | CHEN XiaoBo（Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences,Beijing 100093, China;Graduate University of Chinese Academy of Sciences, Beijing 100039, China） ;
ZHAO XiaoHui（State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China） ;
ZHANG JianPing（State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China） ;
LI LiangBi（Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences,Beijing 100093, China） ;
KUANG TingYun（Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences,Beijing 100093, China） ; |
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| 基金项目： | Supported by the National Natural Science Foundation of China (Grant Nos.39890390 and 20673144) |
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| 摘 要： | The singlet excited state lifetime of the chlorophyll a (Chi a) in cytochrome b6f (Cyt b6f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b6f complexes from different sources (～200 and ～600 ps are the two measured results). The present study demonstrated that the singiet excited state lifetime is associated with the detergents n-dodecyl-β-D-maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b6f complexes. Compared with the Cyt b6f dissolved in β-OG, the Cyt b6f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ～200 ps, of the Chl a in Cyt b6f complex in DDM is closer to the true in vivo.
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| 收稿时间： | 15 August 2006 |
| 修稿时间： | 2 November 2006 |
The effects of detergents DDM and beta-OG on the singlet excited state lifetime of the chlorophyll a in cytochrome b6f complex from spinach chloroplasts. |
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| Authors: | CHEN XiaoBo ZHAO XiaoHui ZHANG JianPing LI LiangBi & KUANG TingYun |
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| Institution: | (1) Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China;(2) State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080, China;(3) Graduate University of Chinese Academy of Sciences, Beijing, 100039, China |
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| Abstract: | The singlet excited state lifetime of the chlorophyll a (Chl a) in cytochrome b
6
f (Cyt b
6
f) complex was reported to be shorter than that of free Chl a in methanol, but the value was different for Cyt b
6
f complexes from different sources (∼200 and ∼600 ps are the two measured results). The present study demonstrated that the
singlet excited state lifetime is associated with the detergents n-dodecyl-β-D-maltoside (DDM) and n-octyl-β-D-glucopyranoside (β-OG), but has nothing to do with the different sources of Cyt b
6
f complexes. Compared with the Cyt b
6
f dissolved in β-OG, the Cyt b
6
f in DDM had a lower fluorescence yield, a lower photodegradation rate of Chl a, and a shorter lifetime of Chl a excited state. In short, the singlet excited state lifetime, ∼200 ps, of the Chl a in Cyt b
6
f complex in DDM is closer to the true in vivo.
Supported by the National Natural Science Foundation of China (Grant Nos. 39890390 and 20673144) |
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| Keywords: | cytochrome b 6 f detergent chlorophyll a singlet excited state lifetime singlet oxygen |
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