Atomic force microscopy study of the rabbit skeletal muscle ryanodine receptors in different functional states |
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Authors: | Wei Qingqing Cheng Xiaoyang Chen Keying Hu Jun Li Minqian Zhu Peihong |
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Institution: | Laboratory of Neurobiology, Shanghai Institute of Physiology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 200031, Shanghai, China. |
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Abstract: | Atomic force microscope was applied to investigate the effect of extrinsic phospholipid on the structure of rabbit skeletal muscle ryanodine receptor/calcium release channel (RyR1). In addition, in the presence of extrinsic phospholipid, the height and elasticity of the RyR1s in different functional states were also measured. The results indicate: (i) most of the RyR1s showed a normal structure only in the presence of extrinsic phospholipid; (ii) treatment of the RyR1s with AMP and Ca2+ together could increase their Young's Modulus but not change their apparent height; (iii) no detectable change in either height or Young's Modulus of the RyR1s appeared, if the RyR1s were treated with other activators or inhibitors. |
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Keywords: | ryanodine receptor/calcium release channel atomic force microscope elasticity |
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