| The complete genome of Enterovirus 71 China strain |
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| 作者姓名： | 扬帆 金奇 何雅青 李良成 候云德 |
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| 作者单位： | YANG Fan JIN Qi HE YaqingLI Liangcheng HOU YundeState Key Laboratory for Molecular Virology and Genetic Engineering，Beijing 100052，China |
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| 基金项目： | the National Natural Science Foundation of China (Grant No.
39823002). |
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| 摘 要： | Five overlapping clones covering the full genome of Enterovirus 71 China strain SHZH98 were obtained and then the sequences were determined by the chain termination method. It showed that the full length of EV71 SHZH98 genome (not including Poly A tail) is 7408 bp. There are some diversities on the lengths and sequences of 5' UTR and 3' UTR between SHZH98 and the other EV71 strains. In P1 capsid region, which is closely associated with viral immunogenicity, EV71 strain SHZH98 shares the highest homology with Taiwan strains; but in P2 and P3 non-structural gene regions there are higher identities with Coxsakievirus A16 and EV71 strains MS, BrCr than with Taiwan strains. Phylogenetic tree constructed by structural gene region indicates that China strain SHZH98 has a closer relationship with Taiwan strains, however, in the non-coding region it has a closer relationship with Coxsakievirus A16, EV71 strains MS and BrCr. EV71 China strain was analyzed at the molecular level. The results will contribute to the
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| 收稿时间： | 29 September 2000 |
The complete genome of Enterovirus 71 China strain |
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| Fan Yang,Qi Jin,Yaqing He,Liangcheng Li,Yunde Hou.The complete genome of Enterovirus 71 China strain[J].Science China Life sciences,2001,44(2):178-183. |
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| Authors: | Fan Yang Qi Jin Yaqing He Liangcheng Li Yunde Hou |
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| Institution: | (1) State Key Laboratory for Molecular Virology and Genetic Engineering, 100052 Beijing, China |
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| Abstract: | Five overlapping clones covering the full genome of Enterovirus 71 China strain SHZH98 were obtained and then the sequences were determined by the chain termination method. It showed that the full length of EV71 SHZH98 genome (not including Poly A tail) is 7408 bp. There are some diversities on the lengths and sequences of 5' UTR and 3' UTR between SHZH98 and the other EV71 strains. In P1 capsid region, which is closely associated with viral immunogenicity, EV71 strain SHZH98 shares the highest homology with Taiwan strains; but in P2 and P3 non-structural gene regions there are higher identities with Coxsakievirus A16 and EV71 strains MS, BrCr than with Taiwan strains. Phylogenetic tree constructed by structural gene region indicates that China strain SHZH98 has a closer relationship with Taiwan strains, however, in the non-coding region it has a closer relationship with Coxsakievirus A16, EV71 strains MS and BrCr. EV71 China strain was analyzed at the molecular level. The results will contribute to the basic study on enteroviruses and the EV71 prevention in China. |
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| Keywords: | Enterovirus 71 nucleotide sequence analysis homology analysis |
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