Nicotinic acetylcholine receptor is involved in acetylcholine regulating stomatal movement |
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Authors: | WANG Hengbin WANG Xuechen ZHANG Shuqiu LOU Chenghou |
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Institution: | (1) College of Biological Sciences, China Agricultural University, 100094 Beijing, China |
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Abstract: | In animal cells, actions of acetylcholine depend on its binding to specific receptors. Activation of nicotinic acetylcholine
receptor can affect the membrane permeability to ions directly. As concerned about acetylcholine regulating stomatal movement,
nicotinic acetylcholine receptor may also be involved. Experiments with nicotinic acetylcholine receptor antagonist (D-tubocurarine)
and agonist (nicotine) showed that nicotinic acetylcholine receptor was involved in acetylcholine inducing stomata1 opening.
In addition, these effects of D-tubocurarine and nicotine on stomatal movement can only be found in medium containing K+ while no effects can be found in medium containing Ca2+, The result by Western blotting showed that at least α-and β-subunits of nicotinic receptor existed in microsomes of guard
cell protoplasts. It was found that the distribution of nicotinic acetylcholine receptor was mainly on the surface of protoplasts
as shown by FITC labelled α-bungarotoxin. The results above show that nicotinic acetylcholine receptor exists in guard cells
and mediates acetylchlone to induce stomatal opening.
Project supported by the National Natural Science Foundation of China (Grant No. 39730050). |
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Keywords: | Vicia faba\% L stomatal movement acetylcholine nicotinic acetylcholine receptor |
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