| Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines |
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| 作者姓名： | 蔡宏 田霞 呼西旦 潘怡 李国利 庄玉辉 朱玉贤 |
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| 作者单位： | National Laboratory of Protein Engineering and Plant Genetic Engineering，College of Life Science，Peking University，National Laboratory of Protein Engineering and Plant Genetic Engineering，College of Life Science，Peking University，Xinjiang Academy of Animal Science，Institute of Veterinary，National Laboratory of Protein Engineering and Plant Genetic Engineering，College of Life Science，Peking University，Tuberculosis Research Laboratory，309 Hospital of PLA，Tuberculosis Research Laboratory，309 Hospital of PLA，National Laboratory of Protein Engineering and Plant Genetic Engineering，College of Life Science，Peking University Beijing 100871，China，Beijing 100871，China，Urumqi 830000，China，Beijing 100871，China，Beijing 100091，China，Beijing 100091，China，Beijing 100871，China |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | Tuberculosis is an ancient scourge of mankind. According to statistics, there are more than 10 million new cases of tuberculosis each year and the annual death toll for tuberculosis exceeds three millions. The current available BCG is of questionable efficacy, and its protection ranges from 0 to 85%. Therefore, developing a safe and effective vaccine against this scourge is very important. Previous studies have shown that the secreted proteins of Mycobacterium tuberculosis (M. TB) can induc…
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| 收稿时间： | 2002-08-30 |
| 修稿时间： | 2002-12-25 |
Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines |
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| CAI Hong,TIAN Xia,HU Xidan,PAN Yi,LI Guoli,ZHUANG Yuhui,ZHU Yuxian.Immunogenicity and protective efficacy study using combination of four tuberculosis DNA vaccines[J].Science China Life sciences,2003,46(5):495-502. |
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| Authors: | CAI Hong TIAN Xia HU Xidan PAN Yi LI Guoli ZHUANG Yuhui ZHU Yuxian |
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| Institution: | (1) National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Science, Peking University, 100871 Beijing, China;(2) Xinjiang Academy of Animal Science, Institute of Veterinary, 830000 Urumqi, China;(3) Tuberculosis Research Laboratory, 309 Hospital of PLA, 100091 Beijing, China |
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| Abstract: | Immune response and protective efficacy for the combination of four tuberculosis DNA vaccines were evaluated in this study. We obtained 1:200 antibody titers against Ag85B 21d after mice were vaccinated for the first time by four recombinant eukaryotic expression vectors containing coding sequences for Ag85B, MPT-64, MPT-63 and ESAT-6. The titers of Ag85B were elevated to 1:102400 after the second injection and decreased to 1:12800 after the third injection. Antibody titers for MPT-64 and MPT-63 reached 1:25600 21 d after the first vaccination, and were then decreased following the second and third injections. No antigen-specific antibody titer against ESAT-6 was detected in sera harvested from immunized mice at any time. These DNA vaccines evoked specific IFN-λ responses in the spleens of vaccinated mice as well. When challenged with M. tuberculosis H37Rv, we found that the lungs of the vaccinated mice produced 99.8% less bacterial counts than that of the empty-vector control group and the bacterial counts were also significantly less than that of the BCG group. Histopathological analyses showed that the lungs of vaccinated mice produced no obvious caseation while over 50%-70% of the pulmonary parenchyma tissue produced central caseation in the vector control group. Our results indicated that the combination of four tuberculosis DNA vaccines may generate high levels of immune responses and result in better animal protection. |
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| Keywords: | Mycobacterium tuberculosis secreted proteins combination DNA vaccines immunogenicity protective efficacy |
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