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Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane
作者姓名:朱勇  韩学海  杨福愉
作者单位:National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China
基金项目:Project supported by the National Natural Science Foundation of China (Grant No. 39730130).
摘    要:Cytochrome c is a component of mitochondrial respiratory chain, located at the outer side of mitochondrial inner membrane. Its precursor, apocytochrome c, is encoded by a nuclear gene, synthesized on cytoplasmic ribosomes, and posttranslationally imported into mitochondria, but apocytochrome c is unique in the translocation compared with most mitochondrial proteins. It does not carry a cleavable amino terminal targeting sequence; no proteinous receptor on the mitochondrial outer membrane is identified for its import and its translocation does not compete with other preproteins for translocation machinery in the outer membrane. Besides, neither ATP nor membrane potential is required for its translocation across mitochonctria.

收稿时间:10 April 1998

Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane
ZHU Yong,HAN Xuehai,YANG Fuyu,.Translocation of chicken heart apocytochrome c and its mutants (C17S, H18D) across mitochondrial membrane[J].Science China Life sciences,1999,42(1):1-7.
Authors:ZHU Yong  HAN Xuehai  YANG Fuyu  
Institution:(1) National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China
Abstract:The dependence of import of chicken heart apocytochrome c on its transformation to holoform by heme attachment was studied. Results showed that there was no difference in the translocation of apocytochrome c across the mitochondrial membrane in the presence or absence of hemin dithionite. Furthermore, two heme unattached mutants (H18D, C17S) were prepared, which could still be accumulated in mitochondria, but their import velocity was obviously reduced.
Keywords:chicken heart apocytochrome c  mutants H18D  C17S  translocation across mitochondrial membrane  
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