| Preliminarily functional analysis of a cloned novel human gene ADAM29 |
| |
| 作者姓名： | 王昉 徐人尔 朱鹏程 胡俊杰 应蓓蓓 赵寿元 李昌本 |
| |
| 作者单位： | WANG Fang XU Rener ZHU PengchengHU Junjie YING Beibei ZHAO Shouyuan& LI ChangbenSchool of Life Science，Institute of Genetics，State Key Laboratory of Genetic Engineering，Fudan University，Shanghai 200433，China |
| |
| 基金项目： | the Life Science Center of Shanghai. |
| |
| 摘 要： | ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis andcontains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of tes-tis-associated cells.
|
Preliminarily functional analysis of a cloned novel human |
| |
| WANG Fang,XU Rener,ZHU PengchengHU Junjie,YING Beibei,ZHAO Shouyuan& LI ChangbenSchool of Life Science.Preliminarily functional analysis of a cloned novel human gene ADAM29[J].Science China Life sciences,2001(4). |
| |
| Authors: | WANG Fang XU Rener ZHU PengchengHU Junjie YING Beibei ZHAO Shouyuan& LI ChangbenSchool of Life Science |
| |
| Institution: | WANG Fang XU Rener ZHU PengchengHU Junjie YING Beibei ZHAO Shouyuan& LI ChangbenSchool of Life Science,Institute of Genetics,State Key Laboratory of Genetic Engineering,Fudan University,Shanghai 200433,China |
| |
| Abstract: | ADAM is a family of type I integral membrane proteins which are characterized by sharing a disintegrin and metalloprotease domain and involved in many important physiological processes such as fertilization, neurogenesis and inflammatory response. A novel human ADAM gene--ADAM29, which was cloned in our laboratory, is exclusively expressed in human testis andcontains a potential fusion domain. A full-length cDNA of ADAM29 was obtained by using multiple-step PCR. Phylogenetic tree of known mammalian ADAMs specifically expressed in testis was reconstructed. Polyclonal antiserum was raised by immunizing the rabbits with sub-peptide of ADAM29 (Leu268-Asp374) as immunogen. The result of immunohistochemical test on human testis showed that ADAM29 is expressed in different stages of spermatogenesis and in interstitial cells. ADAM29 may play a certain role in the signal transduction during the maturation of tes-tis-associated cells. |
| |
| Keywords: | ADAM family ADAM29 phylogenetic tree immunohistochemistry testis interstitial cell |
| 本文献已被 CNKI 等数据库收录！ |
