Crystal structure determination of basic phospholipase A2 from venom of Agkistrodon halys pallas by molecular replacement method |
| |
Authors: | MENG Wuyi LIN ZhengjiongZHOU Yuancong |
| |
Institution: | 1.State Key Laboratory of Biomacromolecules; Institute of Biophysics; Chinese Academy of Sciences; Beijing 100101; China
2.Shanghai Institute of Biochemistry; Shanghai 200032; China |
| |
Abstract: | Basic phospholipase A2 (BPLA2) from the venom of Agkistrodon halys pallas has a strong ability to hemolyze erythrocytes. The asymmetrical unit of P212121 crystal of BPLA2 contains two molecules. Self-rotation function was used to study the orientation relationship of these two molecules. Cross-rotation and translation functions were then used to determine the orientations and positions of the two molecules in the unit cell. The model building and preliminary structure refinement were carried out. The result shows that the two molecules in the asymmetrical unit of orthorhombic crystal are related by a non-crystallographic 2-fold symmetry axis. |
| |
Keywords: | phospholipase A2 from Agkistrodon halys pallos hemolysis molecular replacement method crystal structure |
|
| 点击此处可从《中国科学:生命科学英文版》浏览原始摘要信息 |
| 点击此处可从《中国科学:生命科学英文版》下载免费的PDF全文 |