| A new method of preparing fiber-optic DNA biosensor and its array for gene detection |
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| 作者姓名： | 姜广奋 陈润生 阎宏 欧阳颀 |
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| 作者单位： | JIANG Guangfen CHEN Runsheng VAN Hong& OUYANG QiInstitute of Biophysics，Chinese Academy of Sciences，Beijing 100101，China;Department of Physics，Peking University，Beijing 100871，China |
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| 摘 要： | A new method of preparing fiber-optic DNA biosensor and its array for the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-optic DNA biosensors by adsorbing and immobilizing the oligonucleotide probe on its end. By assembling the fiber-optic DNA biosensors in a bundle in which each fiber carried a different DNA probe, the fiber-optic DNA biosensor array was well prepared. Hybridization of fluorescent- labeled cDNA of p53 gene, N-ras gene and Rb1 gene to the DNA array was monitored by CCD camera. A good result was achieved.
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| 收稿时间： | 30 March 2000 |
A new method of preparing fiber-optic DNA biosensor and its array for gene detection |
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| Guangfen Jiang,Runsheng Chen,Hong Yan,Qi Ouyang.A new method of preparing fiber-optic DNA biosensor and its array for gene detection[J].Science China Life sciences,2001,44(1):33-39. |
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| Authors: | Guangfen Jiang Runsheng Chen Hong Yan Qi Ouyang |
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| Institution: | (1) Institute of Biophysics, Chinese Academy of Sciences, 100101 Beijing, China;(2) Department of Physics, Peking University, 100871 Beijing, China |
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| Abstract: | A new method of preparing fiber-optic DNA biosensor and its array for the simultaneous detection of multiple genes is described. The optical fibers were first treated with poly-l-lysine, and then were made into fiber-optic DNA biosensors by adsorbing and immobilizing the oligonucleotide probe on its end. By assembling the fiber-optic DNA biosensors in a bundle in which each fiber carried a different DNA probe, the fiber-optic DNA biosensor array was well prepared. Hybridization of fluorescent- labeled cDNA of p53 gene, N-ras gene and Rb1 gene to the DNA array was monitored by CCD camera. A good result was achieved. |
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| Keywords: | fiber-optic DNA biosensor DNA array gene detection |
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