| Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum) |
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| 作者姓名： | 龙鸿 曾宪录 焦明大 胡波 孙海晶 刘振兰 张立勇 郝水 |
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| 作者单位： | Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University，Institute of Genetics and Cytology，Northeast Normal University Changchun 130024，China，Changchun 130024，China，Changchun 130024，China，Changchun 130024，China，Changchun 130024，China，Changchun 130024，China，Changchun 130024，China，Changchun 130024，China |
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| 基金项目： | the Major State Basic Research Program (973) of China. |
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| 摘 要： |
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Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum) |
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| LONG Hong,ZENG Xianlu,JIAO Mingda,HU Bo,SUN Haijing,LIU Zhenlan ,ZHANG Liyong, & HAO Shui.Analysis of nucleolar pre-rRNA processing sites in pea(Pisum sativum)[J].Science China Life sciences,2003,46(1). |
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| Authors: | LONG Hong ZENG Xianlu JIAO Mingda HU Bo SUN Haijing LIU Zhenlan ZHANG Liyong & HAO Shui |
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| Institution: | email: ycsuo@nenu.edu.cn |
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| Abstract: | The location of rRNA processing was analyzed by using in situ hybridization with ITS1 probe and immunolabeling of anti-fibrillarin mAb in pea (Pisum sativum) root pole cells. The results showed that rRNA processing sites were in dense fibrillar components (DFCs) and granular com-ponents (GCs), but not in fibrillar centers (FCs). Low doses of actinomycin D (AMD) treatment can selectively suppress pre-rRNA synthesis but cannot disturb the processing of preformed pre-rRNAs. With AMD treatment prolonged, the density of labeled signals gradually decreased, indicating the preformed pre-rRNAs were gradually processed. |
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| Keywords: | Pisum sativum nucleolus rRNA processing fibrillar centres dense fibrillar components granular compo-nents |
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