(1) Department of Physics, University of Science and Technology of China, 230026 Hefei, China;(2) Department of Biology, University of Science and Technology of China, 230026 Hefei, China
Abstract:
Laser tweezers can manipulate small particles, such as cells and organelles. When coupling them with laser microbeam selective fusion of two tobacco protoplasts containing some chloroplast was achieved. Physical and biological variables that affect laser trapping and laser-induced fusion were also discussed. The results show that the effect of chloroplast content and distribution on the yield of cell fusion is remarkable.