| Simultaneous multi-parameter observation of Harring-tonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy |
| |
| 作者姓名： | 张春阳 李艳平 马辉 李素文 薛绍白 陈瓞延 |
| |
| 作者单位： | ZHANG Chunyang LI Yanping MA HuiLI Suwen XUE Shaobai CHEN DieyanMolecular and Nano Sciences Laboratory，Department of Physics，Tsinghua University，Beijing 100084，China;Department of Biology，Beijing Normal University，Beijing 100875，China |
| |
| 基金项目： | the Tsinghua University Foundation for Basic Research and the Chinese Postdoctoral Foundation. |
| |
| 摘 要： | Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simultaneously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration (Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell.
|
| 收稿时间： | 25 December 2000 |
Simultaneous multi-parameter observation of Harringtonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy |
| |
| Chunyang Zhang,Yanping Li,Hui Ma,Suwen Li,Shaobai Xue,Dieyan Chen.Simultaneous multi-parameter observation of Harring-tonine-treating HL-60 cells with both two-photon and confocal laser scanning microscopy[J].Science China Life sciences,2001,44(4):383-391. |
| |
| Authors: | Chunyang Zhang Yanping Li Hui Ma Suwen Li Shaobai Xue Dieyan Chen |
| |
| Institution: | (1) Molecular and Nano Sciences Laboratory, Department of Physics, Tsinghua University, 100084 Beijing, China;(2) Department of Biology, Beijing Normal University, 100875 Beijing, China |
| |
| Abstract: | Harringtonine (HT), a kind of anticancer drug isolated from Chinese herb-Cephalotaxus hainanensis Li, can induce apoptosis in promyelocytic leukemia HL-60 cells. With both two-photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, tetramethyrhodamine ethyl ester (TMRE) and Fluo 3-AM, we simultaneously observed HT-induced changes in nuclear morphology, mitochondrial membrane potential and intracellular calcium concentration (Ca2+]i) in HL-60 cells, and developed a real-time, sensitive and invasive method for simultaneous multi-parameter observation of drug- treating living cells at the level of single cell. |
| |
| Keywords: | two-photon laser scanning microscopy confocal laser scanning microscopy Harringtonine HL-60 cells |
| 本文献已被 CNKI SpringerLink 等数据库收录！ |
| 点击此处可从《中国科学：生命科学英文版》浏览原始摘要信息 |
| 点击此处可从《中国科学：生命科学英文版》下载免费的PDF全文 |
