Isolation of pea matrix attachment region and study on its function in transgenic tobaccos |
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Authors: | Xugang Li Zhen Zhu Junwang Xu Qian Wu Honglin Xu |
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Institution: | (1) Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China |
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Abstract: | A DNA fragment containing consensus sequence of matrix attachment region (MAR) has been isolated from pea genome. Compared
with original DNA sequence, one 115 bp-long repeat sequence is deleted in the obtained DNA sequence. DNA fragments located
upstream and downstream of repeat DNA sequence respectively share 84% and 93% homology to the corresponding original sequence,
and contain A-box or T-box and TATAA sequence, which is characteristics short sequence of MARs. To test the function of the
DNA sequence, the plant expression vectors in which β-glucuronidase gene (GUS, uidA) was used as reporter gene were constructed
and transferred into tobaccosvia Agrobacterium- mediated transformation procedure. Quantitative GUS assay showed that the average level of uidA expression was increased
twofold for the presence of MAR, and the highest level of GUS activity of transgenic plants could be increased six times.
The results cited above suggest that the isolated DNA sequence contains consensus sequence of MARs and has capability to increase
expression level of gene in transgenic plants. |
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Keywords: | matrix attachment region transgenic plants β -glucuronidase foreign gene expression |
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