Preliminary investigation of sequence-independent DNA binding proteins in rat skeletal muscle sarcoplasmic reticulum and their function |
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| Authors: | Wen Zhao Zhisheng Jiang Juhua Ni Kuanghueih Chen Naikui Liu Jian Tang Hongti Jia Chaoshu Tang |
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| Institution: | (1) Institute of Cardiovascular Research, Beijing Medical University, 100083 Beijing, China;(2) Institute of Cardiovascular Research, The First Hospital of Beijing Medical University, 100034 Beijing, China;(3) Department of Biochemistry and Molecular Biology, Beijing Medical University, 100083 Beijing, China |
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| Abstract: | To observe the binding of plasmid DNA to non-nuclear DNA binding proteins in sar-coplasmic reticulum (SR) and the effects of this binding on SR function, sarcoplasmic reticulum proteins in rat skeletal muscle were isolated by differential centrifuge and sucrose density-gradient centrifuge. The results showed that there are two sequence-independent DNA binding proteins in SR proteins, the molecular weights of which are 83 and 58 ku, respectively. Ca2 uptake and release of SR were remarkably promoted by the binding of plasmid DNA to DNA binding proteins in SR, the mechanism is probably through increasing of Ca2 -ATPase activity in SR and changing of character of Ca2 release channel ryanodine receptors induced by the binding. These results suggest that there exist DNA binding proteins in SR and its binding to DNA may affect Ca2 transport of SR. |
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| Keywords: | DNA binding proteins of SR Ca2 uptake and release Ca2 -ATPase ryanodine receptor |
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