| Fine-mapping of an Arabidopsis cell death mutation locus |
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| 作者姓名： | 牟中林 戴亚 李家洋 |
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| 作者单位： | Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China
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| 摘 要： | An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region.
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| 收稿时间： | 16 June 1999 |
Fine-mapping of an Arabidopsis cell death mutation locus |
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| Zhonglin Mou,Ya Dai,Jiayang Li.Fine-mapping of an Arabidopsis cell death mutation locus[J].Science China Life sciences,2000,43(2):138-145. |
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| Authors: | Zhonglin Mou Ya Dai Jiayang Li |
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| Institution: | (1) Plant Biotechnology Laboratory, Institute of Genetics, Chinese Academy of Sciences, 100101 Beijing, China |
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| Abstract: | An Arabidopsis cell death mutation locus was mapped to chromosome 2 between lGS1 and mi421. The YAC clone ends, CIC9A3R, CIC11C7L, CIC2G5R and RFLP marker CDs3 within this interval, were used to probe TAMU BAC library and 31 BAC clones were obtained. A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes. 11 CAPS and 12 STS markers were developed in this region. These results will facilitate map-based cloning of the genes and sequencing of the genomic DNA in this region. |
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| Keywords: | Arabidopsis cell death mod1 mutant physical map CAPS marker |
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