| Assembly characteristics of plant keratin intermediate filaments in vitro |
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| 作者姓名： | 闵光伟 杨澄 佟向军 翟中和 |
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| 作者单位： | College of Life Sciences，Peking University，Beijing 100871，China; Department of Biological Science and Technology，Tsinghua University，Beijing 100084，China |
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| 基金项目： | Project supported by the National Natural Science Foundation of China (Grant No. 39370352)，the Doctor Foundation of Ministry of Education of China. |
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| 摘 要： | After selective extraction and purification, plant keratin intermediate filaments were reassembled in vitro. Scanning tunneling microscope (STM) and transmission electron microscope (TEM) micrographs showed that acidic keratins and basic keratins can assemble into dimers and further into 10 nm filaments in vitro. In higher magnification images, it can be seen that fully assembled plant keratin intermediate filaments consist of several thinner filaments of 3 nm in diameter, which indicates the formation of protofilaments in the assembly processes. One of the explicit features of plant keratin intermediate filaments is a 24—25 nm periodic structural repeat alone the axis of beth the 10 nm filaments and protofilaments. The periodic repeat is one of the fundamental characteristic of all intermediate filaments, and demonstrates the half staggered arrangement of keratin molecules within the filaments.
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Assembly characteristics of plant keratin intermediate filaments in vitro |
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| MIN Guangwei YANG Cheng TONG Xiangjun,and ZHAI Zhonghe!Corresponding author.Assembly characteristics of plant keratin intermediate filaments in vitro[J].Science China Life sciences,1999(5). |
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| Authors: | MIN Guangwei YANG Cheng TONG Xiangjun and ZHAI Zhonghe!Corresponding author |
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| Abstract: | After selective extraction and purification, plant keratin intermediate filaments were reassembled in vitro. Scanning tunneling microscope (STM) and transmission electron microscope (TEM) micrographs showed that acidic keratins and basic keratins can assemble into dimers and further into 10 nm filaments in vitro. In higher magnification images , it can be seen that fully assembled plant keratin intermediate filaments consist of several thinner filaments of 3 nm in diameter, which indicates the formation of protofilaments in the assembly processes. One of the explicit features of plant keratin intermediate filaments is a 24-25 nm periodic structural repeat alone the axis of both the 10 nm filaments and protofilaments. The periodic repeat is one of the fundamental characteristic of all intermediate filaments, and demonstrates the half staggered arrangement of keratin molecules within the filaments. |
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| Keywords: | intermediate filament keratin in vitro assembly scanning tunneling microscope (STM) |
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