A Rapid Method for Preparing Undecalcified Sections of Bone for Autoradiographic Investigation with Short-Lived Radionuclides |
| |
| Authors: | T J F Savelkoul W J Visser J M M Roelofs M H F Lentferink |
| |
| Institution: |
a Clinical Researchgroup for Bone Metabolism, Department of Internal Medicine, University Hospital, P.O. Box 16250, 3500 CG, Utrecht, The National Institute of Public Health, Bilthoven, The Netherlands |
| |
| Abstract: | To prepare sections of undecalcified bone suitable both for autoradiography with short-lived radionuclides such as 99m-technetium (t1/2 = 6 hr) and for normal histology, rapid processing is necessary. By modifying the routine technique of embedding in plastic, sections can be obtained within 6 hours. The most important modification concerns the temperature used for the different steps in the process. The procedure has been used to localize 99mTc labeled methylene diphosphonate for skeletal scintigraphy. |
| |
| Keywords: | |
| 本文献已被 InformaWorld 等数据库收录！ |
|
