Basic Blue 148: A Rapid Stain for T Helper Cells |
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Authors: | Lawrence Kass |
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Institution: |
a Departments of Pathology and Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio |
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Abstract: | After brief exposure to an aqueous solution of the oxazine textile dye C. I. basic blue 148 following fixation in 37% formalin, 95% ethanol and glacial acetic acid, T helper cell nuclei and cytoplasm in specimens of peripheral blood displayed a deep red-violet color. No other cell in normal blood or bone marrow specimens showed intense staining of this type. The total staining time is 1 min. Basic blue 148 stain is a promising technique for hematology and immunology laboratories as a rapid screening test for T helper cells in blood specimens using a microscopic slide and ordinary incandescent illumination. |
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Keywords: | oxazine dye T helper cells lymphocytes light microscopy |
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