A Membrane Filter Mounting Method and Spore Stain for Saccharomyces |
| |
Authors: | J J Miller V V Kingsley |
| |
Institution: |
a Department of Biology, Hamilton College, McMaster University, Hamilton, Ontario, Canada |
| |
Abstract: | Sporulated yeast cells seeded on 5 × 10 mm rectangles of ultrathin Millipore membrane filter are stained through the membrane with Ziehl's carbol-fuchsins. This is followed by 0.1% aqueous ruthenium red which both destains and counterstains. The preparations are made permanent with a water mounting medium. By a modification of the procedure microcolonies can be grown on the membranes, stained, and permanently mounted. |
| |
Keywords: | |
本文献已被 InformaWorld 等数据库收录! |
|