Three-Way Differentiation of Chinese Hamster Ovary Chromosomes by Immunoperoxidase Technique Using a Monoclonal Anti-Bromodeoxyuridine Antibody |
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| Authors: | J Pi ero T Ortiz F Cort s |
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| Institution: |
a Department of Cell Biology, University of Seville, Seville, Spain |
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| Abstract: | A new permanent staining procedure for sister chromatid differentiation (SCD) in cultured Chinese hamster ovary cells has been established by combining the three-way differentiation in third mitosis (M3) chromosomes and the immunoperoxidase reaction developed with 3,3'-diaminobenzidine using a monoclonal anti-bromodeoxyuridine (BrdU) antibody. This procedure allows SCD at very low BrdU concentrations, and the evaluation of the sister chromatid exchange frequencies on a per cell-cycle basis. |
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| Keywords: | bromodeoxyuridine (BrdU) monoclonal anti-BrdU antibody sister chromatid exchange (SCE) differential staining |
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