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A Rapid Method for Microbial Sample Preparation for the Scanning Electron Microscope
Authors:Sushama Chaphalkar  Rajdeep Dongre  Deepa Joshi  Sabita Dey
Institution:  a Department of Microbiology, Maharashtra Association for the Cultivation of Science, Research Institute, Pune, India b Electron Microscopy Laboratory, Maharashtra Association for the Cultivation of Science, Research Institute, Pune, India
Abstract:A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms.
Keywords:SEM  microorganism  spores
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