A Rapid Method for Microbial Sample Preparation for the Scanning Electron Microscope |
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Authors: | Sushama Chaphalkar Rajdeep Dongre Deepa Joshi Sabita Dey |
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Institution: |
a Department of Microbiology, Maharashtra Association for the Cultivation of Science, Research Institute, Pune, India
b Electron Microscopy Laboratory, Maharashtra Association for the Cultivation of Science, Research Institute, Pune, India |
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Abstract: | A synthetic aromatic polymer has been used for preparing replicas of different microorganisms. This method of preparing highly concentrated (9.6 k) microbiological samples for scanning electron microscopy was compared with a standard method. The micrographs of the replicated samples are satisfactory. This method is rapid, cost effective and produces good results, especially in the case of spore-forming mycelial microorganisms. |
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Keywords: | SEM microorganism spores |
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