Epigenetic and phenotypic changes result from a continuous pre and post natal dietary exposure to phytoestrogens in an experimental population of mice |
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Authors: | Carlos M Guerrero-Bosagna Fernanda S Valdovinos Luis E Valladares Susan J Clark |
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Institution: | 1.Center for Reproductive Biology, School of Molecular Biosciences, Washington State University,Pullman,USA;2.Laboratorio de Ecofisiología Animal, Departamento de Ciencias Ecológicas, Facultad de Ciencias,Universidad de Chile,Santiago,Chile;3.Center for Advanced Studies in Ecology & Biodiversity and Departamento de Ecología, Facultad de Ciencias Biológicas,Pontificia Universidad Católica,de Chile,Chile;4.Laboratorio de Hormonas y Receptores,Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile,Santiago,Chile;5.Epigenetics Laboratory, Cancer Program,Garvan Institute of Medical Research,Sydney,Australia |
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Abstract: | Background Developmental effects of exposure to endocrine disruptors can influence adult characters in mammals, but could also have evolutionary
consequences. The aim of this study was to simulate an environmental exposure of an experimental population of mice to high
amounts of nutritional phytoestrogens and to evaluate parameters of relevance for evolutionary change in the offspring. The
effect of a continuous pre- and post-natal exposure to high levels of dietary isoflavones was evaluated on sexual maturity,
morphometric parameters and DNA methylation status in mice. Adult mice male/female couples were fed ad libitum either with control diet (standard laboratory chow) or ISF diet (control diet plus a soy isoflavone extract at 2% (w/w) that
contained the phytoestrogens genistein and daidzein). In the offspring we measured: i) the onset of vaginal opening (sexual
maturation) in females, ii) weight and size in all pups at 7, 14, 21 and 42 days post-natal (dpn) and iii) DNA methylation
patterns in skeletal α-actin (Acta1), estrogen receptor-α and c-fos in adults (42 dpn). |
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