中国白块菌——攀枝花块菌子囊果内可培养细菌的多样性研究

对新近发现的块菌属一新种——攀枝花白块菌（Tuber panzhihuanense）子囊果中可培养细菌的多样性进行了研究。采用胰蛋白大豆培养基（TSA）对菌株进行分离。用毛细管电泳（HPCE）对所有获得的菌株的16S rDNA V3高变区进行筛选获得不同条带大小的菌株，对筛选出的菌株的16S rDNA进行测序，并进行细菌多样性分析和研究。结果显示，攀枝花块菌子囊果内可培养细菌在数量及种类上都表现出很高的多样性，所有细菌分属于5个门的11个属和20个种。在所分离到的变形菌门的细菌中，数量最多的菌株（4968%）属于γ Proteobacteria，其中假单胞菌属的Pseudomonas lurida为优势类群；其次为α Proteobacteria，占3742%，其中以固氮菌 Bradyrhizobium japonicum和Phyllobacterium spp.为优势类群。其余的菌株属于放线菌门（Actinobacteria） （322%）和厚壁菌门（Firmicutes） （774%），厚壁菌门中以芽孢杆菌属（Bacillus）为代表菌群。酸杆菌门中的Terriglobus roseus（194%）首次从块菌中分离获得。

Diversity of Culturable Bacteria Associated with Ascocarps of a Chinese White Truffle,Tuber panzhihuanense (Ascomycota)*

Culturable bacterial communities inhabiting ascocarps of Tuber panzhihuanense were investigated. Isolates obtained on tryptone soy agar (TSA) were screened with high performance capillary electrophoresis (HPCE) according to differences in size of 16S rDNA V3. Target isolates were identified by analysis of the whole length of 16S rDNA gene. The results revealed that the ascocarps of T. panzhihuanense harbored a great number of culturable bacteria which belonging to 20 species and 11 genera in 5 phyla. Most isolates (4968%) were affiliated to the γ Proteobacteria, dominated by Pseudomonas lurida. The second major subclass was α Proteobacteria (3742%), with Phyllobacterium and a nitrogen fixing bacterium Bradyrhizobium japonicum also occurring as dominant taxa. The remaining bacterial isolates contained members of Actinobacteria (322%) and Firmicutes (774%) of which Bacillus was the commonest bacterium. A novel Tuber associated culturable bacterium species, Terriglobus roseus, was isolated and detected for the first time in Tuber ascocarps.