利用RAPD 技术分析云南兰属部分种的种间关系

采用RAPD 技术对云南兰属的11 个种和3 个变种中的39 个样本进行了相似性分析。在相似系数0. 58 水平上, 大花亚属的虎头兰、西藏虎头兰、长叶兰和碧玉兰聚为一支; 建兰亚属的寒兰、墨兰、蜜蜂兰、蕙兰、春兰、豆瓣兰、春剑、莲瓣兰和送春聚为另一支, 兔耳兰与大花亚属关系更近。春剑和莲瓣兰相似性更高, 它们与春兰的关系较远, 不支持送春作为蕙兰下的变种。这些结果可为开展兰属育种提供重要参考价值。

Similarity Analysis of the Main Species of Cymbidium (Orchidaceae) from Yunnan Province Using RAPD Technique

39 accession of 11 species and 3 varieties of Cymbidium in Yunnan Province were analyzed in similarity by using RAPD technique . Subgenus Cyperorchis, such as C. hookerianum, C. tracyanum, C. erythraeum, and C. lowianum areclustered into a clade ; Subgenus Jensoa , including C. kancan , C. sinense, C. pumilum, C. faberi , C. goeringii, C. goeringii var. serratum, C. tortisepalum var. longibracteatum, C. tortisepalum and C. cyperifolium var . szechuanicum are classified into another clade . C. lancifolium is closer to subgenus Jensoa than to the others . C. tortisepalum var . longibracteatum is close to C. tortisepalum var. tortisepalum and both of them are far from C. goeringii . It is not supported that C. cyperifolium var. szechuanicum belongs to a variety of C. faberi . These findings may benefit to Cymbidium breeding.