多胺对离体黄瓜子叶花芽构建的影响*

研究了外源多胺对离体黄瓜( Cucumis sativus L. ) 子叶内在多胺含量和花芽构建的影响。在MS 培养
基中添加5@ 10- 2 mmol#L- 1 亚精胺( Spd) 、精胺( Spm) 能明显提高子叶内Spd 含量和SpdPPut 值, 子叶成
花率也明显高于对照组, 而添加5 @ 10- 2 mmol#L- 1 Put、5 mmol#L- 1 环己胺硫酸盐( CHAS) 培养的结果则相
反。表明子叶内Spd 含量和SpdPPut 与成花率呈正相关关系。在添加5 mmol#L- 1 D- 精氨酸( D-Arg) 处理后,
子叶内SpdPPut 高于Spd 处理, 但子叶成花率低于对照组, 表明Put、Spd 含量也影响离体子叶花芽的构建。
在实验中添加CHAS 处理后子叶内Spm 含量高出对照组10 倍, 子叶成花率却为0, 其它各处理与对照组的内
源Spm 变化基本一致, 成花率却差别明显, 表明子叶内Spm 含量与黄瓜子叶花芽构建之间无明显相关性。

The Effect of Polyamines on the Floral Bud Construction of in vitro Cotyledons in Cucumis sativus ( Cucurbitaceae)

The effects of exogenous polyamines on the levels of polyamines in the in vitro cotyledons and on the floral bud
construction were examined in Cucumis sativus . Application of 5 @ 10- 2 mmol#L- 1 spermidine ( Spd) and spermine
( Spm) inMS culture medium remarkably increases the levels of Spd and the ratio of SpdPputrescine ( Put) in cotyledons
and results in the rate of floral buds higher than that of control; however, application of 5@ 10- 2 mmol#L- 1 Put or 5 mmol#L- 1
CHAS leads to opposite results, suggesting that the levels of Spd and ratio of SpdPPut in cotyledons are positive correlated
with the rate of floral buds. Application of 5mmol#L- 1 D-Arg in medium results in that the ratio of SpdPPut is higher than
that treated with Spd and that rate of floral buds in cotyledons is lower than that of control, indicating that the levels of Put
and Spd have effects on the construction of floral buds. Comparing with control, application of CHAS increases the levels
of Spm in cotyledons 10 folds and decreases the rate of floral buds to zero. There is no significant difference in the levels
of endogenous Spm between other treatments and control, except the rate of floral buds, meaning there is no correlation
between the levels of Spm in cotyledons and the construction of floral buds in Cucumis sativus .