Chromosomal mapping of T-DNA inserts in transgenicPetunia byin situ hybridization |
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| Authors: | J Wang M E Lewis J H Whallon K C Sink |
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| Institution: | (1) Department of Horticulture, Michigan State University, 48824 East Lansing, MI, USA;(2) Department of Crop and Soil Sciences, Michigan State University, 48824 East Lansing, MI, USA;(3) Present address: Pioneer Hi-Bred International, Inc., 50131 Johnston, IA, USA |
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| Abstract: | The chromosomal location of T-DNa inserts in ten independently derived and confirmed transgenic plants ofP. hybrida was detected byin situ hybridization. Nine transgenic plants had the T-DNA inerts at single sites distributed among each of the seven chromosomes; in one plant the T-DNA inserts were detected on two different chromosomes. Although the T-DNA inserts were integrated randomly among the chromosomes, seven of the 11 total inserts were located at or near the telomere. Thus, T-DNA inserts appear to have potential for tagging chromosomes and chromosome fragments. |
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| Keywords: | Petunia T-DNA in situ hybridization tagging |
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