Expression of Functional Interleukin-12 from Mouse in Transgenic Tomato Plants |
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| Authors: | Abel?Gutiérrez-Ortega Claudia?Sandoval-Montes Teresa?Jesús?de?Olivera-Flores Leopoldo?Santos-Argumedo Email author" target="_blank">Miguel?ángel?Gómez-LimEmail author |
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| Institution: | (1) Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del I.P.N., Unidad Irapuato. Km 9.6 Libramiento Norte Carretera Irapuato-León, Apdo., Postal 629, 36500 Irapuato, Guanajuato, México;(2) Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados del I.P.N., Av. I.P.N. 2508, 07360 Col. San Pedro Zacatenco, D.F., México;(3) Facultad de Química, Conjunto E, Laboratorio 116, Paseo de la Investigación Científica, Universidad Nacional Autónoma de México, 04510, DF.CP, México |
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| Abstract: | Transgenic plants have been employed successfully as a low-cost system for the production of therapeutically valuable proteins,
including antibodies, antigens and hormones. Here, we report the expression of a cytokine with immunomodulatory function,
mouse interleukin-12 (IL-12), in transgenic tomato plants. Single-chain mouse IL-12 driven by the CaMV 35S promoter, accumulates
to high levels in leaves and fruits (up to 7.3 and 3.4 μg per gram of fresh weight, respectively). Mouse IL-12 expressed in
tomato displays biological activity in vitro, as determined by interferon-γ (IFN-γ) secretion by T cells. Possible uses of this plant-based cytokine involving mucosal
delivery are discussed |
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| Keywords: | interferon gamma interleukin-12 recombinant proteins tomato transgenic plants |
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