人血浆低密度脂蛋白亚组分氧化反应敏感性的比较

本文对３种ＬＤＬ亚组分在体外对Ｃｕ＾２＋催化氧化反应敏感性进行了比较。结果表明，随氧化时间延长，各ＬＤＬ亚组分的电泳迁移率均增加。测定脂质过氧化物的含量以及用结合二烯法测定氧化反应的潜伏期，发现较高密度的ＬＤＬ亚组分更易氧化。荧光免疫测定结果显示，较高密度ＬＤＬ中载脂蛋白Ｂ上新生的４－羟壬烯醛抗原决定簇的表达高于较低密度的ＬＤＬ，从而证明较高密度的ＬＤＬ亚组分对氧化反应的敏感性高于较低密度的亚组分

Comparison between the Susceptibility of Human Plasma Low Density Lipoprotein Subfractions to Oxidation

The susceptibility of three low density lipoprotein (LDL) subfractions to in vitro Cu2+-catalyzed oxidation was compared. The results demonstrated that 1)the reltive electrophoretic mobilities of all oxidized LDL subfractions were increased with the oxidation time; 2) by measuring the lipid peroxides content and the lag phase of conjugated dienes formation, the LDL subfractions of higher density were more susceptible to oxidative modification; and 3) the expression of 4hydroxynonenal-derived epitopes on apolipoprotein B was higger in denser LDLsubfractions. Thus, we conclude that the denser LDL subfractions are more susceptible to oxidation.