| 重组人血清白蛋白在Pichia pastoris中的表达与纯化 |
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| 引用本文： | 邱荣德,李士云.重组人血清白蛋白在Pichia pastoris中的表达与纯化[J].生物化学与生物物理学报,2000,32(1):59-62. |
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| 作者姓名： | 邱荣德 李士云 |
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| 作者单位： | 中国科学院上海生物化学研究所,上海 |
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| 摘 要： | 为实现重组人血清白蛋白（ｒＨＳＡ）的开发，对构建的酵母工程菌Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ ＧＳ１１５／ＨＳＡ进行了表达条件的优化，摇瓶中将表达ｒＨＳＡ的量提高到１５０ｍｇ／Ｌ。经中空纤维柱浓缩、Ｐｈｅｎｙｌ－Ｓｅｐｈａｒｏｓｅ分离和抗ＨＳＡ－Ｓｅｐｈａｒｏｓｅ亲和层析纯化获得电泳纯的重组人血清白蛋白。
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| 关 键 词： | rHSA Pichia pastoris 基因表达 人血清白蛋白 |
High Expression and Purification of Recombinant Human Serum Albumin from Pichia pastoris |
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| QIU Rong De,LI Shi Yun,CHEN Jun Gang,WU Xiang Fu,YUAN Zhong Yi.High Expression and Purification of Recombinant Human Serum Albumin from Pichia pastoris[J].Acta Biochimica et Biophysica Sinica,2000,32(1):59-62. |
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| Authors: | QIU Rong De LI Shi Yun CHEN Jun Gang WU Xiang Fu YUAN Zhong Yi |
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| Abstract: | Recombinant human serum albumin (rHSA) was produced in methylotrophic yeast Pichia pastoris . By optimization of expression, about 150 mg/L of rHSA was obtained from broth of Pichia pastoris GS115/ HSA (his Mut S) supernatant. The rHSA was isolated and purified by hollow fiber ultrafiltration, Phenyl Sepharose hydrophobic chromatography and antibody immunoadsorbent chromatography. Finally, rHSA was purified to electrophoretic purity. |
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| Keywords: | rHSA Pichia pastoris gene expression isolation and purification |
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