鹅副粘病毒SF02 F基因的序列分析及SF02的多重RT—PCR鉴别

对新近分离的鹅副粘病毒SF0 2采用RT PCR方法 ,扩增F基因后测序 ,得到全长的F基因。该基因的ORF总长为 16 6 2nt,编码 5 5 3个氨基酸 ,其裂解位点的序列为112 R R Q K R F117,与新城疫病毒强毒株的特征相符。其核苷酸和氨基酸同源性分析 ,并与国内新城疫病毒标准强毒株F4 8E9相比较 ,表明该毒株在F基因上已发生了较大的变异 ,而与近年来在我国台湾和部分西欧国家流行的禽副粘病毒有很高的亲缘关系。在分析F基因序列的基础上 ,设计 3条引物 ,建立了一种新的多重RT PCR方法 ,能区分鸡新城疫病毒与鹅副粘病毒。

Sequence Analysis of F Gene of SF02 Isolate of Goose Paramyxovirus and Its Identification by Multiplex RT PCR

The complete F gene of SF02 of goose paramyxovirus (GPV) has been cloned and analyzed. The sequence analysis demonstrated that the F gene of SF02 contains 1 662 nt and encodes 553 amino acids, and its cleavage activation site of F gene has the same deduced amino acid sequence, 112 R R Q K R F 117 , as the velogenic (highly pathogenic) strain of newcastle disease virus. The latter correlated with the virulence of the isolate in biological assays. The F gene of SF02 isolate with the domestic standard velogenic strain NDV, F48E9, shared 86.5% homology in nucleotide and 90.8% homology in amino acid sequences. The SF02 isolate is closer to some NDV strains prevalent in Taiwan and West European countries in recent years. Based on the F gene sequence a multiplex RT PCR method has been developed. It could be used for the discrimination of GPV from NDV.