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EF—Tumt和EF—Tsmt在不同发育阶段小鼠各组织中的表达分析
引用本文:陈Li 许青.EF—Tumt和EF—Tsmt在不同发育阶段小鼠各组织中的表达分析[J].生物化学与生物物理学报,1999,31(1):37-40.
作者姓名:陈Li  许青
作者单位:上海医科大学医学神经生物学国家重点实验室
基金项目:国家自然科学基金,国家教委留学回国人员科研启动基金,卫生部科学研究基金
摘    要:线粒体蛋白质翻译延长因子Tu和Ts(mitochondrialelongationfactorTuandTs,EFTumtandEFTsmt)是由核基因编码的两个蛋白质,它们的功能和调控对细胞的生长发育有重要意义。采用EFTumt和EFTsmt重组蛋白分别制备了抗EFTumt和抗EFTsmt特异抗体并以此检测了它们在小鼠不同发育时期心肌、骨骼肌、肝、脑、脾等组织中的表达。蛋白质印迹结果表明EFTumt和EFTsmt在各组织中的表达水平不同、有明显的组织差异性,并都受发育的调节。EFTumt在同一发育时期各组织中的表达及随发育的变化趋势与EFTsmt基本一致。结果提示EFTumt和EFTsmt的表达水平与组织细胞能量代谢水平密切相关,它们不仅在体内以复合体形式发挥作用,其基因表达可能受同一机制的调控。

关 键 词:基因表达  EF-Tumt  EF-Tsmt  发育阶段  小鼠

Differential and Tissue specific Expression of Mitochondrial Elongation Factor Tu and Ts during Development
CHEN Li,XU Qing,LIU Xu Lei,ZHAO Jing and MA Lan.Differential and Tissue specific Expression of Mitochondrial Elongation Factor Tu and Ts during Development[J].Acta Biochimica et Biophysica Sinica,1999,31(1):37-40.
Authors:CHEN Li  XU Qing  LIU Xu Lei  ZHAO Jing and MA Lan
Abstract:The mitochondrial elongation factors Tu and Ts (EF Tu mt and EF Ts mt ) are nuclear gene encoded mitochondrial proteins and play important roles in growth and development. We prepared antibodies against EF Tu mt and EF Ts mt using purified recombinant EF Tu mt and EF Ts mt . Western analysis indicates EF Tu mt and EF Ts mt are expressed differently in various tissues. Their expression in heart and liver increased during development and reached plateau at maturation. Expression of EF Tu mt and EF Ts mt exhi bited similar tissue specificity and changed synchronically during development. These results suggest that expression levels of EF Tu mt and EF Ts mt may be regulated by a similar mechanism related to the metabolic state of the cells.
Keywords:Mitochondrial elongation factor Tu and Ts  gene expression  
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