| 蜂毒肽在磷脂膜上取向的高效液相色谱和质谱研究 |
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| 引用本文： | 牛葳,武轶.蜂毒肽在磷脂膜上取向的高效液相色谱和质谱研究[J].生物化学与生物物理学报,1999,31(4):390-394. |
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| 作者姓名： | 牛葳 武轶 |
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| 作者单位： | 清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室!北京100084，清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室!北京100084，2北京微量化学研究所，北京100091，清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室!北京100084，清华大学生物科学与技 |
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| 摘 要： | 利用脂质体模型系统研究了跨膜电位对蜂毒肽在磷脂磷上取向的影响，采用高效液相色谱与质谱技术相结合方法，分析了蜂毒肽与磷脂膜作用后的胰蛋白酶酶解产物，结果发现，当蜂毒肽分子与没有跨膜电位的脂质体结合后，它的所有酶切位点都能够酶有效切断，当蜂毒肽与带负向膜电全的脂质体结合后，其Ｎ端的一个酶切位点被封闭，从而说明跨膜电位造成了蜂毒肽在膜上的重瓣取向。
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| 关 键 词： | 蜂毒肽 磷脂膜 膜上取向 跨膜电位 HPLC 质谱 |
HPLC and MS Studies of Orientation of Melittin on Phospholipid Membrane |
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| NIU Wei ,WU Yi ,YANG Yu Feng and SUI Sen Fang.HPLC and MS Studies of Orientation of Melittin on Phospholipid Membrane[J].Acta Biochimica et Biophysica Sinica,1999,31(4):390-394. |
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| Authors: | NIU Wei WU Yi YANG Yu Feng and SUI Sen Fang |
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| Institution: | NIU Wei 1,WU Yi 1,2*,YANG Yu Feng 1 and SUI Sen Fang 1 |
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| Abstract: | The orientation of melittin binding to liposomes with or without transmembrane potential was investigated. With a combination of high performance liquid chromatography and mass spectrometry, the membrane association state of melittin was detected by analyzing its trypsin digested products. It was found that the enzyme could access all the proteolytic sites on protein binding to the liposome without membrane potential, while one proteolytic site on the N terminal of the protein was blocked after it binding to the liposome with negative transmembrane potential. The results showed that melittin changed its orientation in the latter case. |
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| Keywords: | Melittin orientation on membranes transmembrane potential high performance liquid chromatography(HPLC) mass spectrometry(MS) |
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