| 端粒酶RNA的反义cDNA对乳腺癌细胞端区长度的影响 |
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| 引用本文： | 张晓伟 童坦君. 端粒酶RNA的反义cDNA对乳腺癌细胞端区长度的影响[J]. Acta biochimica et biophysica Sinica, 1999, 31(5): 527-530 |
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| 作者姓名： | 张晓伟 童坦君 |
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| 作者单位： | 北京医科大学生物化学与分子生物学系!北京100083 |
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| 摘 要： | 应用反义核酸技术，将端粒酶ＲＮＡ的ｃＤＮＡ反向插入整合型腺病毒载体ｐＡｄＥ１ＣＭＶＩＴＲＥＸｎｅｏ，与ｐＢ－ＨＧ１１Ｘ共转染２９３细胞反获得反义重组病毒。将此反义重组病毒感染乳腺癌细胞ＭＣＦ－７后，使细胞端区长度缩短。
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| 关 键 词： | 端粒酶 反义cDNA 端区 乳腺癌 |
Telomere Shortening of MCF 7 Cells Caused by Antisense Telomerase cDNA |
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| ZHANG Xiao Wei,LIAO Hai Ni and TONG Tan Jun. Telomere Shortening of MCF 7 Cells Caused by Antisense Telomerase cDNA[J]. Acta biochimica et biophysica Sinica, 1999, 31(5): 527-530 |
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| Authors: | ZHANG Xiao Wei LIAO Hai Ni TONG Tan Jun |
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| Abstract: | pAdE 1CMVITREX neo is a novel adenovirus vector which can integrate foreign genes into genome of host cells. The cDNA of human telomerase RNA was integrated inversely into the vector via antisense oligonucleotide technique. Antisense recombinant virus (vAdT AAV) was obtained by cotransfecting pAdT and pBHG 11 into 293 cells. The telomere length of breast cancer MCF 7 cells was found shortened after the transfection by vAdT AAV. |
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| Keywords: | telomerase antisense cDNA telomere |
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